Abstract:
Global interest in using duckweed (Lemna minor) as a substitute for fish, livestock, and human diets has spurred research on the
mass culture of the species. There is a scarcity of information on the L. minor fatty acid composition in different aquaculture
settings. A comparative study was carried out to investigate the fatty acid composition of L. minor cultured in indoor plastic tanks
and outdoor earthen ponds for 30 days. During the culture period, culture facilities were fertilized using livestock manure. Fatty
acid characterization was done using gas chromatography method. Fifteen fatty acids: five saturated (SAFAs), five monounsaturated
(MUFAS), and five polyunsaturated (PUFAs) were identified. Fatty acid compositions varied between indoor and outdoor
settings. Percentage composition of L. minor PUFAs cultured outdoor (37.13) was higher than that of indoor (21.96) settings.
L. minor SAFAs percentage composition was higher in the indoor culture at 41.63% while that of outdoor was 33.75%. The
composition of L. minor MUFAs in indoor tanks was higher (36.32%) than in outdoor earthen ponds (29.10%). This study
indicated the presence of docosahexaenoic, eicosapentaenoic, linoelaidic, and eicosanoic acids in L. minor seldom reported in
past studies. Paired Students’ t-test indicated that the means of the fatty acid composition were significantly different (p<0:05) in
both settings, with docosahexaenoic showing the highest paired mean difference.
